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KMID : 1059519970410120672
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Journal of the Korean Chemical Society
1997 Volume.41 No. 12 p.672 ~ p.676
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Solubilization of Brain Phospholipase D by Taurodeoxycholate: Activational Effect of Some Matal Ions
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Choi Seok-Woo
Choi Myung-Un
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Abstract
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Microsomal phospholipase D (PLD) in rat brain was solubilized employing 0.2 % taurodeoxycholate in high ionic strength. Phopholipase D activity was determined by measuring product phophatidic acid (PA) using isotope-labelled dipalmitoylphophatidylcholine as a substrate. The solubilized PLD showed an optimal pH of 6.5 and the highest activity at 30 ℃. These properties were similar to those of microsomal PLD before solubilization. The stimulatory effect of oleic acid was observed at the concentration of 4 mM. When effects of metal ions on PLD activity were examined, alkaline earth metals such as Mg2+, Ca2+, Sr2+, Ba2+ promoted the PA production but Cu2+, Cd2+, Al3+, Ni2+, V5+ showed inhibitory effects.
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